AnaPath offers intracellular investigations within the nanoscale range using in-house Transmission Electron Microscope (TEM). The microscopy can be extended with Energy Dispersive X-Ray Spectrometry (EDS/EDX) services.
Our microscope (Hitachi HT7820) allows to observe the ultrastructure of the samples because of the high magnification range (50 – 800 000 x) and its lateral resolution of 0.14 nm. The energy dispersive X-ray spectrometer (Bruker) allows investigation of the chemical composition of biological samples.
TEM uses high-energy electrons to penetrate through the sample and highly sensitive detectors to detect the transmitted electrons and create the image. It allows us to observe the inner structure of the sample with much higher resolution than standard light microscopy.
TEM provides information of the cellular ultrastructure within the nanometer range. It can detect even the smallest intracellular changes the EDS analysis allows to investigate the chemical structure within the cell.
Our TEM is equipped with Energy Dispersive X-Ray Spectrometer (EDS/EDX) that is one of the most widely used analytical techniques. The spectrometer detects characteristic X-rays emitted from the material and uses them for the elemental analysis of the sample.
That is very helpful for example for toxicological studies where it allows us to find out which elements are in the specific area of the sample.
The application range for the TEM is very large. We can visualize many samples, such as molecules (proteins, lipids, DNA…), viruses, bacteria, tissues, cells… We can examine the ultrastructure of all those samples within a nanometer range. EDS allows to investigate the chemical composition of their inner structure. For more application examples, click here.
For more information and consultation on the different available services, contact us.